Recent publications on molecular methods or data

[Martin Spies]

2015

Failla, A. J., Vasquez, A. A., Hudson, P., Fujimoto, M., Ram, J. L. (2015) Morphological identification and COI barcodes of adult flies help determine species identities of chironomid larvae (Diptera, Chironomidae). Bull. entomol. Res. 106(1): 34-46.

Kranzfelder, P., Ekrem, T., Stur, E. (2015) Trace DNA from insect skins: a comparison of five extraction protocols and direct PCR on chironomid pupal exuviae. Mol. Ecol. Resources, Early View [First published online, DOI: 10.1111/1755-0998.12446]

Krosch, M. N., Bryant, L. M. (2015) A note on sampling chironomids for RNA-based studies of natural populations that retains critical morphological vouchers. CHIRONOMUS J. Chironomidae Res. 28: 4-11.

Tischler A., Egg, M., Füreder, L. (2015) Ethanol: A simple and effective RNA-preservation for freshwater insects living in remote habitats. Limnol. Oceanogr.: Methods [first publ. online, doi: 10.1002/lom3.10079 (xi.2015), 10 pp.].

[Martin Spies]

2016

Augustyniak, M., Gladysz, M., Dziewięcka, M. (2016) The Comet assay in insects—Status, prospects and benefits for science. Mutation Res. – Revs Mut. Res. 767: 67-76.

Hambäck, P. A., Weingartner, E., Dalén, L., Wirta, H., Roslin, T. (2016) Spatial subsidies in spider diets vary with shoreline structure: Complementary evidence from molecular diet analysis and stable isotopes. Ecology and Evolution 6(23): 8431-8439 + online suppl. info.

Kuncham, R., Thayumanavan, T., Reddy, G. V. S. (2016) Development of a modified mitochondrial DNA extraction method from chironomid larvae. J. Envir. Biol. 37(3): 473-478.

Meier, R., Wong, W. H., Srivathsan, A., Foo, M. S. (2016) $1 DNA barcodes for reconstructing complex phenomes and finding rare species in specimen-rich samples. Cladistics 32(1): 100-110 + 6 online suppl. files.

Pramual, P., Simwisat, K., Martin, J. (2016) Identification and reassessment of the specific status of some tropical freshwater midges (Diptera: Chironomidae) using DNA barcode data. Zootaxa 4072(1): 39-60.

Song, C., Wang, Q., Zhang, R., Sun, B., Wang, X. (2016) Exploring the utility of DNA barcoding in species delimitation of Polypedilum (Tripodura) non-biting midges (Diptera: Chironomidae). Zootaxa 4079(5): 534-550.

[Martin Spies]

2017

Bista, I., Carvalho, G. R., Walsh, K., Seymour, M., Hajibabaei, M., Lallias, D., Christmas, M., Creer, S. (2017) Annual time-series analysis of aqueous eDNA reveals ecologically relevant dynamics of lake ecosystem biodiversity. Nature Communic. 8(14087), 11 pp. + suppl. material [publd online]

Kranzfelder, P., Ekrem, T., Stur, E. (2017) DNA barcoding for species identification of insect skins: a test on Chironomidae (Diptera) pupal exuviae. J. Insect Sci. 17(6, paper 111): 7 pp. + 2 suppl. tables [publd online xii.2017]

Krosch, M. N. (2017) Comparative analysis of larval transcriptomes from co-occurring species of Australian Cricotopus (Diptera: Chironomidae). Austral Ent. 56(4): 451-458 + online suppl. material.

[Martin Spies]

2018

Carew, M. E., Kellar, C. R., Pettigrove, V. J., Hoffmann, A. A. (2018) Can high-throughput sequencing detect macroinvertebrate diversity for routine monitoring of an urban river? Ecol. Indicators 85: 440-450 + online suppl. data.

Elías-Gutiérrez, M., Valdez-Moreno, M., Topan, J., Young, M. R., Cohuo-Colli, J. A. (2018) Improved protocols to accelerate the assembly of DNA barcode reference libraries for freshwater zooplankton. Ecology and Evolution 8(5): 3002-3018 + 6 online suppl. files.

Lin, X.-L., Stur, E., Ekrem, T. (2018) Exploring species boundaries with multiple genetic loci using empirical data from non-biting midges. Zool. Scripta 47: 325-341 + online suppl. material.

Song, C., Lin, X.-L., Wang, Q., Wang, X.-H. (2018) DNA barcodes successfully delimit morphospecies in a superdiverse insect genus. Zool. Scripta 47: 311-324 + online suppl. material. [the genus treated is Polypedilum]

Srivathsan, A., Baloglu, B., Wang, W., Tan, W. X., Bertrand, D., Ng, A. H. Q., Boey, E. J. H., Koh, J. J. Y., Nagarajan, N., Meier, R. (2018) A MinION^TM-based pipeline for fast and cost-effective DNA barcoding. Mol. Ecol. Resources 18(5): 1035-1049 + online suppl. material.

Theissinger, K., Kästel, A., Elbrecht, V., Makkonen, J., Michiels, S., Schmidt, S. I., Allgeier, S., Leese, F., Brühl, C. A. (2018) Using DNA metabarcoding for assessing chironomid diversity and community change in mosquito controlled temporary wetlands. Metabarcoding and Metagenomics 2: e21060; 13 pp. + online suppl. material. [publd online (i.2018); DOI: 10.3897/mbmg.2.21060]

Wang, W. Y., Srivathsan, A., Foo, M., Yamane, S. K., Meier, R. (2018) Sorting specimen-rich invertebrate samples with cost-effective NGS barcodes: Validating a reverse workflow for specimen processing. Mol. Ecol. Resources 18(3): 490-501. [no chironomid content; posted here for method info.]

[Martin Spies]

2019

Czechowski, P., Stevens, M. I., Madden, C., Weinstein, P. (2019) Steps towards a more efficient use of chironomids as bioindicators for freshwater bioassessment: Exploiting eDNA and other genetic tools. Ecol. Indicators 110: 11 pp. + online suppl. material. [first publd online (20.xi.2019)]

Gajski, G., Žegura, B., Ladeira, C., Pourrut, B., Del Bo’, C., Novak, M., Sramkova, M., Milic, M., Gutzkow, K. B., Costa, S., Dusinska, M., Brunborg, G., Collins, A. (2019) The comet assay in animal models: From bugs to whales – (Part 1 Invertebrates). Mutation Res. / Revs Mutation Res. 779: 82-113.

McCulloch, G. A., Purcell, M. F., Harms, N. E., Grodowitz, M. J., Zhang, J., Hong, S.-H., Walter, G. H. (2019) Molecular screening of herbivorous flies collected from Hydrilla verticillata across China and Korea – setting up hypotheses for further exploratory surveys and tests. Biol. Control 138(paper 104051): 6 pp. + online suppl. material.

Morinière, J., Balke, M., Doczkal, D., Geiger, M. F., Hardulak, L. A., Haszprunar, G., Hausmann, A., Hendrich, L., Regalado, L., Rulik, B., Schmidt, S., Wägele, J., Hebert, P. D. (2019) A DNA barcode library for 5,200 German flies and midges (Insecta: Diptera) and its implications for metabarcoding-based biomonitoring. Mol Ecol Resour.: 29 pp. + online suppl. material. [first publd online (12.iv.2019); https://doi.org/10.1111/1755-0998.13022]

Weigand, H., Beermann, A. J., Čiampor, F., Costa, F. O., Csabai, Z. et al. [41 authors in total, incl. several chironomid researchers] (2019) DNA barcode reference libraries for the monitoring of aquatic biota in Europe: Gap-analysis and recommendations for future work. Sci. Total Envir. 678: 499-524 + online suppl. material.

[Martin Spies]

2020

Kotrba, M. (2020) The DNA barcoding project on German Diptera: An appreciative and critical analysis with four suggestions for improving the development and reliability of DNA-based identification. Eur. J. Entomol. 117: 315-327. [publd online 28.vii.2020]

Zhang, L., Yang, J., Li, H., You, J., Chatterjee, N., Zhang, X. (2020) Development of the transcriptome for a sediment ecotoxicological model species, Chironomus dilutus. Chemosphere 244(paper 125541): 12 pp. + online suppl. material.